Peer-Reviewed Journal Details
Mandatory Fields
Bist, I;Song, B;Mosa, IM;Keyes, TE;Martin, A;Forster, RJ;Rusting, JF
2016
March
Acs Sensors
Electrochemiluminescent Array to Detect Oxidative Damage in dsDNA Using [Os(bpy)(2)(phen-benz-COOH)](2+)/Nafion/Graphene Films
Published
21 ()
Optional Fields
TANDEM MASS-SPECTROMETRY DNA-DAMAGE LIQUID-CHROMATOGRAPHY ELECTROGENERATED CHEMILUMINESCENCE ELECTRON-TRANSFER STRESS CANCER CARCINOGENESIS MECHANISMS BIOMARKER
1
272
278
Reactive oxygen species (ROS) oxidize guanosines in DNA to form 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG), a biomarker for oxidative stress. Herein we describe a novel 64-microwell electrochemiluminescent (ECL) array enabling sensitive multiplexed detection of 8-oxodG in dsDNA without hydrolysis. Films of Nafion and reduced graphene oxide containing ECL dye [Os(bpy)(2)(phen-benz-COOH)](2+) (OsNG, {bpy= 2,2'-bipyridine and phen-benz-COOH = (4-(1,10-phenanthrolin-6-yl)benzoic acid)}) were assembled into microwells on a pyrolytic graphite wafer to detect 8-oxodG in oligonucleotides by electrochemiluminescence (ECL). DNA oxidation by Fenton's reagent or by ROS formation during redox cycles involving NADPH, Cu-II, and model metabolites was monitored. UPLC-MS/MS of oxidized DNA samples were used for calibration. Detection limit for the fluidic arrays was one 8-oxodG per 670 intact nucleobases, or 0.15%. The method is sensitive enough to evaluate DNA oxidation from biologically relevant ROS-generating reactions of Cull, NADPH, and model metabolites.
WASHINGTON
2379-3694
10.1021/acssensors.5b00189
Grant Details