Peer-Reviewed Journal Details
Mandatory Fields
Choudhury S.;Fitzhenry L.;White B.;Connolly D.
2016
March
Materials
Polystyrene-co-divinylbenzene polyHIPE monoliths in 1.0 mm column formats for liquid chromatography
Published
5 ()
Optional Fields
Isocratic separation Microbore PolyHIPE Polystyrene Reversed phase LC Silcosteel
9
3
© 2016 by the authors. The reversed phase liquid chromatographic (RP-HPLC) separation of small molecules using a polystyrene-co-divinylbenzene (PS-co-DVB) polyHIPE stationary phases housed within 1.0 mm i.d. silcosteel columns is presented within this study. A 90% PS-co-DVB polyHIPE was covalently attached to the walls of the column housing by prior wall modification with 3-(trimethoxysilyl) propyl methacrylate and could withstand operating backpressures in excess of 200 bar at a flow rate of 1.2 mL/min. Permeability studies revealed that the monolith swelled slightly in 100% acetonitrile relative to 100% water but could nevertheless be used to separate five alkylbenzenes using a flow rate of 40 μL/min (linear velocity: 0.57 mm/s). Remarkable column-to-column reproducibility is shown with retention factor variation between 2.6% and 6.1% for two separately prepared columns.
1996-1944
10.3390/ma9030212
Grant Details