Peer-Reviewed Journal Details
Mandatory Fields
Whitaker, G;Liliquist, A;Pasas, SA;O'Connor, R;Regan, F;Lunte, CE;Smyth, MR
2008
June
Journal of Separation Science
CE-UF method for the separation of anthracycyclines: Application to protein binding analysis in plasma using ultrafiltration
Published
21 ()
Optional Fields
PERFORMANCE LIQUID-CHROMATOGRAPHY INDUCED FLUORESCENCE DETECTION METASTATIC BREAST-CANCER CAPILLARY-ELECTROPHORESIS ANTHRACYCLINE ANTIBIOTICS DRUG-RESISTANCE PAROTID-SALIVA DOXORUBICIN DAUNORUBICIN EPIRUBICIN
31
1828
1833
Anthracyclines are chemotherapeutic drugs that are widely used in the treatment of cancers such as lung and ovarian cancers. The simultaneous determination of the anthracyclines, daunorubicin, doxorubicin and epirubicin, was achieved using CE coupled to LIF, with an excitation and emission wavelength of 488 and 560 nm, respectively. Using a borate buffer (105 mM, pH 9.0) and 30% MeOH, a stable and reproducible separation of the three anthracyclines was obtained. The method developed was shown to be capable of monitoring the therapeutic concentrations (50-50000 ng/mL) of anthracyclines. LODs of 10 ng/mL, calculated at an S/N = 3, were achieved. Using the CE method developed, the in vitro protein binding to plasma was measured by ultrafiltration, and from this investigation the estimated protein binding was determined to be in the range of 77-94%.
WEINHEIM
1615-9306
10.1002/jssc.200700629
Grant Details