O'Reilly, EJ;Conroy, PJ;Hearty, S;Keyes, TE;O'Kennedy, R;Forster, RJ;Dennany, L

2015

January

RSC Advances

Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Published

27 ()

ELECTROGENERATED CHEMILUMINESCENCE BIOSENSOR FRAGMENTS ELECTRODE FILMS

5

67874

67877

This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL(-1) detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

CAMBRIDGE

2046-2069

10.1039/c5ra08450d