Peer-Reviewed Journal Details
Mandatory Fields
Kelly, MC;Whitaker, G;White, B;Smyth, MR
2007
June
Free Radical Biology and Medicine
Nickel(II)-catalysed oxidative guanine and DNA damage beyond 8-oxoguanine
Published
14 ()
Optional Fields
CARCINOGENIC NICKEL COMPOUNDS HYDROGEN-PEROXIDE AQUEOUS-SOLUTION BASE DAMAGE CROSS-LINKS RADICALS 8-HYDROXYDEOXYGUANOSINE MECHANISMS INDUCTION HYDROXYL
42
1680
1689
Oxidative DNA damage is one of the most important and most studied mechanisms of disease. It has been associated with a range of terminal diseases such as cancer, heart disease, hepatitis, and HIV, as well as with a variety of everyday ailments. There are various mechanisms by which this type of DNA damage can be initiated, through radiation and chemical oxidation, among others; however, these mechanisms have yet to be fully elucidated. A HPLC-UV-EC study of the oxidation of DNA mediated by nickel(II) obtained results that show an erratic, almost oscillatory formation of 8-oxoguanine (8-oxoG) from free guanine and from guanine in DNA. Sporadic 8-oxoG concentrations were also observed when 8-oxoG alone was subjected to these conditions. A HPLC-MS/MS study showed the fort-nation of oxidised-guanidinohydantoin (oxGH) from free guanine at pH 11, and the formation of guanidinohydantoin (GH) from DNA at pH 5.5. (c) 2007 Elsevier Inc. All rights reserved.
NEW YORK
0891-5849
10.1016/j.freeradbiomed.2007.02.025
Grant Details