Peer-Reviewed Journal Details
Mandatory Fields
Shakalisava, Y;Regan, F
2006
August
Electrophoresis
Determination of association constants of inclusion complexes of steroid hormones and cyclodextrins from their electrophoretic mobility
Published
13 ()
Optional Fields
PERFORMANCE LIQUID-CHROMATOGRAPHY MICELLAR ELECTROKINETIC CHROMATOGRAPHY ALPHA-CYCLODEXTRIN BETA-CYCLODEXTRIN CAPILLARY-ELECTROPHORESIS ESTROGENIC STEROIDS GAS-CHROMATOGRAPHY BINDING CONSTANTS AQUEOUS-SOLUTION SEPARATION
27
3048
3056
CE estimation of the association constants of several steroid hormones with P-CD and gamma-CD and their hydroxypropyl derivative is presented. Estriol, 17 beta-estradiol, ethynylestradiol, estrone, progesterone, mestranol and norethindrone are among the target analytes. The calculation of the cyclodextrin:analyte association constants were performed from the electrophoretic mobility values of steroids at different concentration of CDs in the run buffer. The reliability of the final data was ensured by employing three different linearisation plots (double reciprocal fit, Y-reciprocal fit and X-reciprocal fit). The highest inclusion affinity of target analytes was observed towards gamma-CD and its hydroxypropyl derivative, which is demonstrated by high association constant values and corresponding good linearity of the plots. The affinity of steroids towards a particular CD type based on physical and structural characteristics is explored.
WEINHEIM
0173-0835
10.1002/elps.200500842
Grant Details