Suman;O'Reilly, E;Kelly, M;Morrin, A;Smyth, MR;Killard, AJ

2011

July

Analytica Chimica Acta

Chronocoulometric determination of urea in human serum using an inkjet printed biosensor

Published

11 ()

AMMONIA SENSOR POLYANILINE FABRICATION ELECTRODE FILMS MICROARRAYS CREATININE PROTEINS PATTERNS DOPANT

697

98

102

A biosensor for the determination of urea in human serum was fabricated using a combination of inkjet printed polyaniline nanoparticles and inkjet printed urease enzyme deposited sequentially onto screen-printed carbon paste electrodes. Chronocoulometry was used to measure the decomposition of urea via the doping of ammonium at the polyaniline-modified electrode surface at -0.3 V vs. Ag/AgCl. Ammonium could be measured in the range from 0.1 to 100 mM. Urea could be measured by the sensor in the range of 2-12 mM (r(2) =0.98). The enzyme biosensor was correlated against a spectrophotometric assay for urea in 15 normal human serum samples which yielded a correlation coefficient of 0.85. Bland-Altman plots showed that in the range of 5.8-6.6 mM urea, the developed sensor had an average positive experimental bias of 0.12 mM (< 2% RSD) over the reference method. (C) 2011 Elsevier B.V. All rights reserved.

AMSTERDAM

0003-2670

10.1016/j.aca.2011.04.036