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Mandatory Fields
Online Article
Parle-McDermott A.
2014
October
Methods to study translated pseudogenes: In vitro translation, fusion with a tag/reporter gene, and complementation assay
Published
1
()
Optional Fields
Cloning DHFR Protein expression Pseudogene Retrogene
© Springer Science+Business Media New York 2014. The technical challenge in proving that a given expressed pseudogene is in fact translated into a functional protein is specificity. To circumvent this challenge, one approach is to use PCR in order to generate a series of clones that allow expression of the protein of interest either native or fused to a tag, which can facilitate purification, detection, and complementation in both bacterial and mammalian cells. This approach allows an assessment of whether a putative pseudogenic protein possesses enzymatic activity, to identify its subcel-lular localization and to test its capacity to complement the parental homologue.
1064-3745
243
252
10.1007/978-1-4939-0835-6_16
Grant Details