Microfluidics is advantageous for immunoassay processing (e.g., reducing immunoreagent consumption/assay times) [1], but the fact that most clinically relevant bioassays are developed for well-plate technologies poses significant challenges for integration within μTAS platforms. Assays developed for 96-well plates must be downscaled and adapted for microfluidic settings, involving difficulties in volume and surface chemistry optimization in addition to the inability for microfluidic detection using conventional analytical approaches. To address the need for performing replicate immunoassays in microfluidic environments, we report on the development of translational, slide-based microfluidic cartridges integrated with specialized detection technologies (fluorescence and colorimetric) that enable miniaturization/ analysis of standard assays. Copyright © (2011) by the Chemical and Biological Microsystems Society.