Conference Publication Details
Mandatory Fields
Dimov N.;Gaughran J.;Clancy E.;Barry T.;Smith T.;Ducree J.
2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Automated on-disc total RNA extraction from whole blood towards point-of-care for early stage diagnostics
Optional Fields
bacterial infections On-disc diagnostics RNA spiked blood three-phase extraction
We present a novel integrated, centrifugo-pneumatic micro-homogenizer (╬╝Homogenizer) for automated sample preparation and total RNA extraction from whole blood. Using a Trizol based protocol, this novel ╬╝Homogenizer efficiently lyses whole blood spiked with E. coli, retains the organic-mixed fraction and yields the aqueous phase with the total RNA content. By the interplay of microfluidic design and a protocol of rotational frequencies, we concatenate (and parallelize) a sequence of five subsequent liquid handling operations that complete in less than 10 minutes. A comparison of the total nucleotide content yields similar performance as conventional, essentially manual off-disc sample preparation methods. ┬ę 2013 IEEE.
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