Conference Publication Details
Mandatory Fields
Dimov N.;Gaughran J.;Auley D.;Boyle D.;Kinahan D.;Ducree J.
Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Centrifugally automated solid-phase purification of RNA
Optional Fields
The purity and integrity of RNA after extraction from a biosample like blood are critical for both downstream analysis and development of on-chip diagnostics. We report for the first time on a fully centrifugally automated solidphase purification of RNA on an integrated microfluidic disc with sequential release of on-board reagents. Building on our earlier work the system successfully concatenates event-triggered valving and centrifugally controlled routing in order to collect purified RNA separately from the aqueous and organic wastes. In this article two-stage purification of RNA and sample conditioning through novel high-frequency release and hybrid event-triggered release valves is demonstrated. © 2014 IEEE.
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