Peer-Reviewed Journal Details
Mandatory Fields
Bruneau J.;Stack E.;O'Kennedy R.;Loscher C.
2012
August
Toxicology in Vitro
Aflatoxins B 1, B 2 and G 1 modulate cytokine secretion and cell surface marker expression in J774A.1 murine macrophages
Published
19 ()
Optional Fields
Aflatoxins Immunomodulation Immunosuppression Inflammation J774A.1 Macrophage
26
5
686
693
Aflatoxins are fungal products which occur in food and feed. They are potent hepatocarcinogens, and are known to cause immunosuppression. We investigated the effect of aflatoxin B 1 (AFB 1), aflatoxin B 2 (AFB 2) and aflatoxin G 1 (AFG 1) exposure, alone and in combination, on the secretion of key pro- and anti-inflammatory cytokines from the murine macrophage cell line, J774A.1. Exposure of macrophages to low doses of aflatoxin (0.01 or 0.1ng/mL) resulted in a statistically significant change in the secretion of a number of cytokines following stimulation with lipopolysaccharide (LPS), a component of Gram-negative bacterial cell walls. Specifically, treatment with AFB 1 or AFB 2 alone significantly decreased (P<0.01) the secretion of the anti-inflammatory cytokine interleukin (IL) 10 (IL-10), while the secretion of the pro-inflammatory cytokine IL-6 was significantly increased (P<0.01). In addition, aflatoxin exposure affected expression levels of key cell surface markers involved in the inflammatory response. Toll-like receptor 2 (TLR2) and Cluster of Differentiation 14 (CD14) expression levels decreased significantly (P<0.01), but Toll-like receptor 4 (TLR4) expression was unaffected. This data provides further insight into the mechanisms by which aflatoxins modulate the host immune response to exert their immunosuppressive activity. © 2012 Elsevier Ltd.
0887-2333
10.1016/j.tiv.2012.03.003
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